Human NT-ProANP Quantikine ELISA Kit
Human NT-ProANP Quantikine ELISA Kit
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Human NT-ProANP Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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DANP00
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Contact your distributor for availability.
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1 Kit /
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human NT-ProANP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human NT-ProANP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human NT-ProANP in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human NT-ProANP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human NT-ProANP showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human NT-ProANP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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1.71
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6.04
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11.1
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1.81
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5.95
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11.5
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Standard Deviation
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0.069
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0.224
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0.583
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0.129
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0.426
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0.842
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CV%
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4
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3.7
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5.3
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7.1
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7.2
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7.3
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Recovery
The recovery of human NT-ProANP spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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104
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87-114
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EDTA Plasma (n=4)
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102
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84-120
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Heparin Plasma (n=4)
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98
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84-111
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Serum (n=4)
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108
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95-122
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human NT-ProANP were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
