Human Nectin-4 Quantikine ELISA Kit
Human Nectin-4 Quantikine ELISA Kit
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Human Nectin-4 Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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DNEC40
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (25 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (13 uL)
Assay Range
39.1 - 2,500 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human Nectin-4.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Nectin-4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Nectin-4 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human Nectin-4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Nectin-4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Nectin-4.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Cell Lysates, Urine
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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163
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425
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1365
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194
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474
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1440
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Standard Deviation
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5.56
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13.9
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32.7
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15.8
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27.9
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65.8
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CV%
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3.4
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3.3
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2.4
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8.1
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5.9
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4.6
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Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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232
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603
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1867
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245
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595
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1801
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Standard Deviation
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7.19
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37.7
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40.1
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18.9
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31.9
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82.3
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CV%
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3.1
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6.3
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2.1
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7.7
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5.4
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4.6
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Recovery
The recovery of human Nectin-4 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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100
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94-110
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EDTA Plasma (n=4)
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89
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80-109
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Heparin Plasma (n=4)
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91
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82-99
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Serum (n=4)
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90
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80-101
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Urine (n=4)
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100
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93-108
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Nectin-4 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
