Canine CCL2/MCP-1 Quantikine ELISA Kit
Canine CCL2/MCP-1 Quantikine ELISA Kit
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Canine CCL2/MCP-1 Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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CACP00
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant canine MCP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Canine MCP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine MCP-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine MCP-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine MCP-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine MCP-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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43
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43
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43
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Mean
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37.1
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144
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632
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42.7
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133
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655
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Standard Deviation
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2.2
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5.8
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22
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5.4
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7.5
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30
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CV%
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5.9
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4
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3.5
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12.6
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5.6
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4.6
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Recovery
The recovery of canine MCP-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Supernates (n=4)
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112
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106-121
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EDTA Plasma (n=4)
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110
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102-116
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Heparin Plasma (n=4)
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103
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96-112
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Serum (n=4)
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93
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84-102
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Linearity
To assess the linearity of the assay, samples containing or spiked with high concentrations of canine MCP-1 in each matrix were diluted with Calibrator Diluent and assayed.
