Mouse Periostin/OSF-2 Quantikine ELISA Kit
Mouse Periostin/OSF-2 Quantikine ELISA Kit
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Mouse Periostin/OSF-2 Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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MOSF20
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse Periostin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Periostin/OSF-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Periostin in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse Periostin and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Periostin. Results obtained using natural mouse Periostin showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Periostin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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40
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40
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40
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Mean
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0.6
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0.833
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4.69
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0.629
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0.867
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4.84
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Standard Deviation
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0.021
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0.035
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0.117
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0.056
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0.067
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0.26
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CV%
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3.5
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4.2
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2.5
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8.9
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7.7
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5.4
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Recovery
The recovery of mouse Periostin spiked to three levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Samples (n=6)
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95
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81-117
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Periostin in each matrix were diluted with Calibrator Diluent and assayed.
