Mouse Clusterin Quantikine ELISA Kit
Mouse Clusterin Quantikine ELISA Kit
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Mouse Clusterin Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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MCLU00
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse Clusterin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse Clusterin Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse Clusterin levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Clusterin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Clusterin showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Clusterin.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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1.35
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8.02
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17.5
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1.36
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7.62
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16.2
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Standard Deviation
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0.086
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0.282
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0.682
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0.119
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0.577
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1.62
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CV%
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6.4
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3.5
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3.9
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8.8
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7.6
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10
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Recovery
The recovery of mouse Clusterin spiked into various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Supernates (n=4)
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101
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90-111
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Cell Lysates (n=4)
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106
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95-117
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Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse Clusterin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
