Human AgRP Quantikine ELISA Kit
Human AgRP Quantikine ELISA Kit
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Human AgRP Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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DAGR00
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (25 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human AgRP
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human AgRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human AgRP in cell culture supernates, plasma, and urine. It contains Sf 21-expressed recombinant human AgRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human AgRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human AgRP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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40
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40
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40
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Mean
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56.6
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144
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262
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45.1
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130
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246
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Standard Deviation
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3.14
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4.86
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7.82
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4.66
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10
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18.1
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CV%
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5.5
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3.4
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3
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10.3
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7.7
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7.4
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Cell Culture Supernates, Urine
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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40
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40
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40
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Mean
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54.3
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143
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257
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43.7
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126
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238
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Standard Deviation
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2.64
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3.88
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5.69
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3.91
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8.24
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16.3
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CV%
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4.9
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2.7
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2.2
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8.9
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6.5
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6.8
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Recovery
The recovery of AgRP spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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106
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95-115
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EDTA Plasma (n=4)
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96
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89-106
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Heparin Plasma (n=4)
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92
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84-103
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of AgRP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
