Human ADAM12 Quantikine ELISA Kit
Human ADAM12 Quantikine ELISA Kit
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Human ADAM12 Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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DAD120
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), Heparin Plasma (25 uL), Urine (50 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Urine)
Specificity
Natural and recombinant human ADAM12. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human ADAM12 immunoassay is a 4.5 hour solid phase ELISA designed to measure human ADAM12 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human ADAM12 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ADAM12 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ADAM12.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma, Urine
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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1.39
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3.57
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6.84
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1.43
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3.62
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6.74
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Standard Deviation
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0.057
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0.131
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0.235
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0.079
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0.145
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0.299
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CV%
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4.1
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3.7
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3.4
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5.5
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4
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4.4
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Recovery
The recovery of human ADAM12 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=8)
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98
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88-105
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Heparin Plasma (n=4)
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97
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92-106
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Serum (n=4)
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98
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89-106
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Urine (n=4)
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99
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91-106
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ADAM12 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
