Human Glypican 3 Quantikine ELISA Kit
Human Glypican 3 Quantikine ELISA Kit
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Human Glypican 3 Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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DGLY30
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Cell Lysates (20 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Glypican 3.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Glypican 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Glypican 3 levels in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human Glypican 3 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Glypican 3 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human Glypican 3 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human Glypican 3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.<br>
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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412
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1208
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2387
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466
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1231
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2359
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Standard Deviation
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12
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48
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79.6
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38.4
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75.3
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143
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CV%
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2.9
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4
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3.3
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8.2
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6.1
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6.1
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Recovery
The recovery of human Glypican 3 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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99
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88-112
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EDTA Plasma (n=4)
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88
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81-95
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Heparin Plasma (n=4)
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89
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81-96
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Serum (n=4)
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89
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82-97
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Glypican 3 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
