Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit
Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit
Human Pro-IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary
货号：DLBP00 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL)
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human pro-IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Pro-IL-1 beta is a sandwich enzyme-linked immunoassay. The capture antibody coated to the microplate is specific for the pro-piece of the molecule (first 116 residues), and the detection antibody is specific for mature IL-1 beta. Thus, the assay does not detect either the pro-piece alone or the mature form alone; it is specific instead for the intact pro-IL-1 beta.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
85.5
|
240
|
432
|
85.1
|
248
|
457
|
|
Standard Deviation
|
4.7
|
11.5
|
20.8
|
6.2
|
14
|
28.3
|
|
CV%
|
5.5
|
4.8
|
4.8
|
7.3
|
5.6
|
6.2
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
126
|
374
|
670
|
121
|
342
|
626
|
|
Standard Deviation
|
6.4
|
15.7
|
34.5
|
8.8
|
17.9
|
34.9
|
|
CV%
|
5.1
|
4.2
|
5.1
|
7.3
|
5.2
|
5.6
|
Recovery
The recovery of pro-IL-1 beta spiked to three different levels throughout the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
108
|
99-115
|
|
EDTA Plasma (n=5)
|
103
|
95-108
|
|
Heparin Plasma (n=5)
|
101
|
92-109
|
|
Serum (n=5)
|
102
|
97-108
|
Linearity
To assess the linearity of the assay, five samples spiked with high concentrations of pro-IL-1 beta were diluted in the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
