Human Kallikrein 3/PSA Quantikine ELISA Kit
Human Kallikrein 3/PSA Quantikine ELISA Kit
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Human Kallikrein 3/PSA Quantikine ELISA Kit Summary
货号：DKK300 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
0.9 - 60 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human KLK3/PSA
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human KLK3/PSA Immunoassay is a 4.5 hour solid phase immunoassay designed to measure KLK3/PSA in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human KLK3/PSA, and antibodies raised against the recombinant protein. Natural human KLK3/PSA showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human KLK3/PSA.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
12.3
|
24.3
|
44.1
|
11.8
|
24.2
|
43.8
|
|
Standard Deviation
|
0.7
|
1.3
|
2
|
0.9
|
0.5
|
0.8
|
|
CV%
|
5.7
|
5.4
|
4.6
|
7.7
|
5.8
|
5.5
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
6.96
|
15.5
|
30
|
7.38
|
16.7
|
33
|
|
Standard Deviation
|
0.5
|
0.7
|
0.9
|
0.5
|
0.9
|
1.6
|
|
CV%
|
7.8
|
4.5
|
3
|
6.4
|
5.3
|
5
|
Recovery
The recovery of KLK3/PSA spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
99
|
93-105
|
|
EDTA Plasma (n=4)
|
100
|
93-103
|
|
Heparin Plasma (n=4)
|
101
|
97-109
|
|
Serum (n=4)
|
102
|
97-109
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of KLK3/PSA were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
