Mouse/Rat HGF Quantikine ELISA Kit
Mouse/Rat HGF Quantikine ELISA Kit
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Mouse/Rat HGF Quantikine ELISA Kit Summary
货号：MHG00 规格:96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma)
Specificity
Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
217
|
452
|
1168
|
218
|
447
|
1107
|
|
Standard Deviation
|
8.6
|
19.7
|
62.2
|
14.9
|
21
|
87.7
|
|
CV%
|
4
|
4.4
|
5.3
|
6.8
|
4.7
|
7.9
|
Recovery
The recovery of HGF spiked into various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=4)
|
98
|
85-106
|
|
Mouse Platelet-poor EDTA Plasma (n=4)
|
104
|
101-106
|
|
Mouse Serum (n=4)
|
96
|
84-112
|
|
Mouse Tissue Homogenates (n=3)
|
87
|
80-101
|
|
Rat Cell Culture Supernates (n=4)
|
102
|
85-114
|
|
Rat Platelet-poor EDTA Plasma (n=4)
|
108
|
100-116
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse/rat HGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
