Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary
Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary
Human CCL3/MIP-1 alpha Quantikine ELISA Kit Summary
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货号：DMA00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Assay Range
31.2 - 1,000 pg/mL (Cell Culture Supernates), 46.9 - 1,500 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human MIP-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MIP-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure MIP-1 alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human MIP-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human MIP-1 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
116
|
442
|
726
|
53.6
|
313
|
623
|
|
Standard Deviation
|
3.3
|
5.9
|
14
|
3.7
|
13.4
|
25.7
|
|
CV%
|
2.8
|
1.3
|
1.9
|
6.9
|
4.3
|
4.1
|
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
140
|
688
|
1084
|
50.7
|
306
|
613
|
|
Standard Deviation
|
12.5
|
60.4
|
55.4
|
5.9
|
15.1
|
37.8
|
|
CV%
|
8.9
|
8.8
|
5.1
|
11.6
|
4.9
|
6.2
|
Recovery
The recovery of MIP-1 alpha spiked to different levels in samples throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
100
|
88-117
|
|
Citrate Plasma (n=5)
|
94
|
85-104
|
|
EDTA Plasma (n=5)
|
96
|
81-109
|
|
Heparin Plasma (n=5)
|
98
|
82-121
|
|
Serum (n=5)
|
95
|
87-113
|
