Human IFN-beta Quantikine ELISA Kit
Human IFN-beta Quantikine ELISA Kit
Human IFN-beta Quantikine ELISA Kit Summary
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货号：DIFNB0
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates)
Specificity
Natural and recombinant human IFN-beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-beta in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
58.3
|
159
|
295
|
56.4
|
160
|
275
|
|
Standard Deviation
|
1.71
|
6.01
|
13.8
|
5.26
|
13
|
21.9
|
|
CV%
|
2.9
|
3.8
|
4.7
|
9.3
|
8.1
|
8
|
Recovery
The recovery of human IFN-beta spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=5)
|
107
|
95-111
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
