Human/Mouse/Rat Activin A Quantikine ELISA Kit
Human/Mouse/Rat Activin A Quantikine ELISA Kit
Human/Mouse/Rat Activin A Quantikine ELISA Kit Summary
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货号：DAC00B
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human, mouse, and rat Activin A
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human/Mouse/Rat Activin A Immunoassay is a 5.0 hour solid-phase ELISA designed to measure Activin A in human, mouse, or rat cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human/mouse/rat Activin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/mouse/rat Activin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed Activin A.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
101
|
295
|
498
|
106
|
308
|
529
|
|
Standard Deviation
|
4.2
|
12.5
|
21.9
|
8.4
|
15.5
|
24.8
|
|
CV%
|
4.2
|
4.2
|
4.4
|
7.9
|
5
|
4.7
|
Recovery
The recovery of human Activin A spiked to three different levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
105
|
93-114
|
|
EDTA Plasma (n=4)
|
110
|
95-115
|
|
Heparin Plasma (n=4)
|
107
|
99-113
|
|
Serum (n=4)
|
109
|
100-115
|
Linearity
To assess the linearity of the assay, human samples containing and/or spiked with high concentrations of Activin A were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
