/

全国统一客服热线:
0755-28715175

产品中心
Product
4产品中心
Porcine TNF-alpha Quantikine ELISA Kit Summary
Porcine TNF-alpha Quantikine ELISA Kit Summary
Porcine TNF-alpha Quantikine ELISA Kit Summary

Porcine TNF-alpha Quantikine ELISA Kit Summary

 详情说明

Porcine TNF-alpha Quantikine ELISA Kit Summary

深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!

货号:PTA00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
5 pg/mL
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant porcine TNF-alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine TNF-alpha in cell culture supernates, serum, and plasma samples. It contains E. coli-expressed recombinant porcine TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately measure recombinant porcine TNF-alpha. Results obtained using natural porcine TNF-alpha show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine TNF-alpha.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 79 260 646 88 279 679
Standard Deviation 4.9 9.2 27 8.8 24 44
CV% 6.2 3.5 4.2 10 8.6 6.5

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 104 358 880 106 348 866
Standard Deviation 7.2 13 36 9.7 32 72
CV% 6.9 3.6 4.1 9.2 9.2 8.3

Recovery

The recovery of porcine TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 99 91-105
EDTA Plasma (n=6) 98 83-111
Heparin Plasma (n=4) 91 82-99
Serum (n=6) 105 91-120

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with various concentrations of porcine TNF-alpha in each matrix were diluted with the appropriate Calibrator Diluent and then assayed.
Porcine TNF-alpha ELISA Linearity

Copyright © 深圳9游总区生物科技有限公司版权所有

扫一扫,加关注

客户服务热线:
0755-33164177
13924667705
18665335907
0755-28715175


线