Endothelin-1 Quantikine ELISA Kit
Endothelin-1 Quantikine ELISA Kit
Endothelin-1 Quantikine ELISA Kit Summary
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货号：DET100
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (75 uL), Serum (75 uL), EDTA Plasma (75 uL), Heparin Plasma (75 uL), Urine (75 uL)
Assay Range
0.4 - 25 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Synthetic and natural Endothelin-1
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Endothelin-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure ET-1 in cell culture supernates, serum, plasma, and urine. It contains synthetic ET-1 and antibodies raised against synthetic ET-1. This immunoassay has been shown to accurately quantitate synthetic and naturally occurring ET-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
3
|
7.34
|
14.7
|
3.05
|
7.43
|
14.4
|
|
Standard Deviation
|
0.12
|
0.17
|
0.28
|
0.231
|
0.438
|
0.759
|
|
CV%
|
4
|
2.3
|
1.9
|
7.6
|
5.9
|
5.3
|
Recovery
The recovery of Endothelin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
99
|
88-108
|
|
Human EDTA Plasma (n=4)
|
93
|
87-102
|
|
Human Heparin Plasma (n=4)
|
93
|
85-106
|
|
Human Serum (n=4)
|
98
|
86-107
|
|
Mouse EDTA Plasma (n=4)
|
99
|
102-107
|
|
Mouse Serum (n=4)
|
94
|
86-104
|
|
Rat EDTA Plasma (n=4)
|
99
|
86-113
|
|
Rat Serum (n=4)
|
98
|
88-113
|
|
Urine (n=4)
|
91
|
85-109
|
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of Endothelin-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
