Human IL-12 p70 Quantikine ELISA Kit
Human IL-12 p70 Quantikine ELISA Kit
Human IL-12 p70 Quantikine ELISA Kit Summary
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货号：D1200
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL), Urine (200 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human IL-12p70
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human IL-12 Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-12 in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-12 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant human IL-12 accurately. Results obtained using natural human IL-12 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-12.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
25.7
|
202
|
436
|
19.9
|
115
|
223
|
|
Standard Deviation
|
1.6
|
4.8
|
10.1
|
1.9
|
8.3
|
13.2
|
|
CV%
|
6.2
|
2.4
|
2.3
|
9.5
|
7.2
|
5.9
|
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
21.2
|
228
|
447
|
21
|
126
|
246
|
|
Standard Deviation
|
0.3
|
3.5
|
4.9
|
1.5
|
5.6
|
8.1
|
|
CV%
|
1.4
|
1.5
|
1.1
|
7.1
|
4.4
|
3.3
|
Recovery
The recovery of human IL-12 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
90
|
81-96
|
|
Citrate Plasma (n=5)
|
90
|
82-94
|
|
EDTA Plasma (n=5)
|
101
|
96-109
|
|
Heparin Plasma (n=5)
|
99
|
89-109
|
|
Serum (n=5)
|
100
|
89-106
|
|
Urine (n=5)
|
89
|
85-92
|
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of IL-12 in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
