Human CD163 Quantikine ELISA Kit
Human CD163 Quantikine ELISA Kit
Human CD163 Quantikine ELISA Kit Summary
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货号：DC1630
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Assay Range
1.6 - 100 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant human CD163
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human CD163 immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD163 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CD163 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD163 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD163.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
20
|
35.1
|
65.6
|
20
|
34.9
|
63.6
|
|
Standard Deviation
|
0.75
|
1.2
|
2.3
|
1.3
|
1.6
|
2.6
|
|
CV%
|
3.8
|
3.4
|
3.5
|
6.7
|
4.6
|
4.1
|
Recovery
The recovery of CD163 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
107
|
101-110
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD163 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
