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Human C-Reactive Protein/CRP Quantikine ELISA Kit
Human C-Reactive Protein/CRP Quantikine ELISA Kit
Human C-Reactive Protein/CRP Quantikine ELISA Kit

Human C-Reactive Protein/CRP Quantikine ELISA Kit

 详情说明

Human C-Reactive Protein/CRP Quantikine ELISA Kit Summary

货号:DCRP00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.022 ng/mL
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human CRP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CRP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CRP in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CRP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CRP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CRP.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 4.79 8.66 18.9 4.84 8.66 17.5
Standard Deviation 0.21 0.33 1.57 0.29 0.61 1.15
CV% 4.4 3.8 8.3 6 7 6.6

Recovery

The recovery of CRP spiked to levels throughout the range of the assay in cell culture media was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 92-110


Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CRP were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human C-Reactive Protein/CRP ELISA Linearity

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