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Human HGF Quantikine ELISA Kit
Human HGF Quantikine ELISA Kit
Human HGF Quantikine ELISA Kit

Human HGF Quantikine ELISA Kit

 详情说明

Human HGF Quantikine ELISA Kit Summary

货号:DHG00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.25 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Citrate Plasma (50 uL)
Sensitivity
40 pg/mL
Assay Range
125.0 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant human HGF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human HGF Immunoassay is a 4.25 or 4.5 hour solid phase ELISA that is designed to measure HGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human pro-HGF and antibodies raised against the recombinant factor and has been shown to quantitate recombinant human HGF accurately. Results obtained using natural HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Serum, EDTA Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 339 999 3759 363 967 3790
Standard Deviation 23.7 56.2 154 25.8 81.2 205
CV% 7 5.6 4.1 7.1 8.4 5.4

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 425 991 3869 409 1058 4058
Standard Deviation 30.2 43.2 234 29.4 75.1 264
CV% 7.1 4.4 6 7.2 7.1 6.5

Recovery

The recovery of HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 92 86-101
Citrate Plasma (n=5) 105 99-114
EDTA Plasma (n=5) 100 85-111
Serum (n=5) 99 91-108

Linearity

To assess the linearity of the assay, five samples of each sample type were spiked with high concentrations of HGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human HGF ELISA Linearity

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