/

全国统一客服热线:
0755-28715175

产品中心
Product
4产品中心
Human Leptin Quantikine ELISA Kit
Human Leptin Quantikine ELISA Kit
Human Leptin Quantikine ELISA Kit

Human Leptin Quantikine ELISA Kit

 详情说明

Human Leptin Quantikine ELISA Kit Summary

货号:DLP00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
7.8 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human Leptin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural human Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 64.5 146 621 65.7 146 581
Standard Deviation 2.14 4.32 20 3.56 6.17 20.6
CV% 3.3 3 3.2 5.4 4.2 3.5

Recovery

The recovery of Leptin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 98 94-102
Citrate Plasma (n=5) 95 87-105
EDTA Plasma (n=5) 99 85-112
Heparin Plasma (n=5) 90 81-100
Serum (n=5) 95 89-109

Linearity

To assess the linearity of the assay, cell culture media samples spiked with high concentrations of Leptin and natural serum/plasma samples were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Leptin/OB ELISA Linearity

深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒,价格优势,货期短,欢迎来电咨询0755-33164177!

Copyright © 深圳9游总区生物科技有限公司版权所有

扫一扫,加关注

客户服务热线:
0755-33164177
13924667705
18665335907
0755-28715175


线