Human Kallikrein 5 Quantikine ELISA Kit
Human Kallikrein 5 Quantikine ELISA Kit
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Human Kallikrein 5 Quantikine ELISA Kit
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Catalog #
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Availability
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Size / Price
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DKK500
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Contact your distributor for availability.
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1 Kit /
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (13 uL), Human Milk (13 uL)
Assay Range
78.1 - 5,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk)
Specificity
Natural and recombinant human KLK5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human KLK5 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human KLK5 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human KLK5 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human KLK5 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human KLK5.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
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2
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3
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n
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20
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20
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20
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20
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20
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20
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Mean
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510
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1685
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3407
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577
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1941
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3852
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Standard Deviation
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35.8
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57.2
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75.4
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63.6
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161
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278
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CV%
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7
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3.4
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2.2
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11
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8.3
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7.2
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Recovery
The recovery of KLK5 spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
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97
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91-109
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EDTA Plasma (n=4)
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90
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82-95
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Heparin Plasma (n=4)
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91
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86-103
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Serum (n=4)
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93
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85-106
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of KLK5 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
