Human Pappalysin-1/PAPP-A Quantikine ELISA Kit
Human Pappalysin-1/PAPP-A Quantikine ELISA Kit
Human Pappalysin-1/PAPP-A Quantikine ELISA Kit Summary
货号：DPPA00 规格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human PAPP-A
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human PAPP-A Immunoassay is a 5.5 hour sandwich-type solid phase ELISA designed to measure human PAPP-A in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PAPP-A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PAPP-A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PAPP-A.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
5.49
|
17.4
|
32.7
|
5.2
|
16.4
|
31.4
|
|
Standard Deviation
|
0.32
|
0.78
|
1.39
|
0.44
|
1.14
|
2.04
|
|
CV%
|
5.8
|
4.5
|
4.3
|
8.5
|
6.9
|
6.5
|
