Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit
Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit
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Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit Summary
货号：DBCHE0 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (50 uL), Urine (50 uL), Human Milk (50 uL)
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)
Specificity
Natural and recombinant human BCHE.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Butyrylcholinesterase/BCHE Immunoassay is a 4.5 hour solid phase ELISA designed to measure BCHE levels in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human BCHE and antibodies raised against the recombinant protein. Results obtained for naturally occurring human BCHE showed linear curves that were parallel to the standard curves obtained using the Quantikine Human BCHE Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human BCHE.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
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Intra-Assay Precision
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Inter-Assay Precision
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Sample
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1
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2
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3
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1
|
2
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3
|
|
n
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20
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20
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20
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20
|
20
|
20
|
|
Mean
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1.25
|
2.41
|
5.01
|
1.26
|
2.51
|
5.11
|
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Standard Deviation
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0.031
|
0.044
|
0.154
|
0.063
|
0.114
|
0.177
|
|
CV%
|
2.5
|
1.8
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3.1
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3.1
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4.5
|
3.5
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Recovery
The recovery of human BCHE spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Cell Culture Media (n=4)
|
99
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94-105
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Cell Lysis Buffer 1 (n=2)
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99
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96-102
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Urine (n=4)
|
97
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93-102
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human BCHE were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
