Mouse LIX Quantikine ELISA Kit
Mouse LIX Quantikine ELISA Kit
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Mouse LIX Quantikine ELISA Kit Summary
货号：MX000 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (13 uL), Platelet-poor Heparin Plasma (13 uL), Urine (13 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse LIX
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse CXCL5/LIX Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIX levels in cell culture supernates, tissue lysates, serum, platelet-poor plasma, and urine. It contains E. coli-expressed recombinant mouse LIX and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LIX accurately. Results obtained using natural mouse LIX showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LIX.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
42.7
|
133
|
407
|
41.8
|
120
|
393
|
|
Standard Deviation
|
1.72
|
5.93
|
10.9
|
2.81
|
4.64
|
20.3
|
|
CV%
|
4
|
4.5
|
2.7
|
6.7
|
3.9
|
5.2
|
Recovery
The recovery of mouse LIX spiked to three levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Samples (n=4)
|
114
|
105-120
|
|
Platelet-poor EDTA Plasma (n=4)
|
100
|
92-109
|
|
Platelet-poor Heparin Plasma (n=4)
|
91
|
85-99
|
|
Serum (n=4)
|
87
|
82-94
|
|
Tissue Homogenates (n=4)
|
100
|
87-113
|
|
Urine (n=4)
|
91
|
84-96
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIX were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
