Human ACE Quantikine ELISA Kit
Human ACE Quantikine ELISA Kit
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Human ACE Quantikine ELISA Kit Summary
货号：DACE00 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Saliva (25 uL)
Assay Range
0.8 - 50 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human ACE. In addition to soluble ACE, this kit recognizes membrane-bound forms from lysates of cultured cells and supernates.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human ACE Immunoassay is a 4.5 hour solid phase ELISA designed to measure ACE in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed ectodomain of recombinant human sACE, and antibodies raised against the recombinant factor. Natural human ACE showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human ACE.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Saliva
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
3.6
|
10.7
|
22.1
|
3.44
|
10.3
|
21.8
|
|
Standard Deviation
|
0.08
|
0.3
|
0.95
|
0.21
|
0.4
|
0.87
|
|
CV%
|
2.2
|
2.8
|
4.3
|
6.1
|
3.9
|
4
|
Serum, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
3.57
|
10.9
|
22.5
|
3.77
|
11.7
|
24.4
|
|
Standard Deviation
|
0.12
|
0.43
|
0.9
|
0.29
|
0.6
|
1.2
|
|
CV%
|
3.4
|
3.9
|
4
|
7.7
|
5.1
|
4.9
|
Recovery
The recovery of ACE spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
98
|
89-106
|
|
Heparin Plasma (n=4)
|
100
|
94-106
|
|
Serum (n=4)
|
99
|
91-106
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of ACE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
