Human C-Peptide Quantikine ELISA Kit
Human C-Peptide Quantikine ELISA Kit
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Human C-Peptide Quantikine ELISA Kit Summary
货号：DICP00 规格:96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
Assay Range
28.9 - 1,850 pmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and synthetic human Insulin C-Peptide.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Insulin C-Peptide Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human Insulin C-Peptide in cell culture supernates, serum, plasma, and urine. It contains synthetic human Insulin C-Peptide and has been shown to accurately quantitate the peptide. Results obtained using natural human Insulin C-Peptide showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Insulin C-Peptide.
Precision
Intra-Assay Precision (Precision within an assay) Intra-assay Precision (Precision within an assay)<br>Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Inter-assay Precision (Precision between assays)<br>Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
129
|
436
|
845
|
127
|
437
|
861
|
|
Standard Deviation
|
5.77
|
14.3
|
13.2
|
12.4
|
33
|
65.3
|
|
CV%
|
4.5
|
3.3
|
1.6
|
9.8
|
7.6
|
7.6
|
Recovery
The recovery of human Insulin C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Media (n=4)
|
99
|
84-106
|
|
EDTA Plasma (n=4)
|
101
|
95-108
|
|
Heparin Plasma (n=4)
|
102
|
90-115
|
|
Serum (n=4)
|
103
|
96-109
|
|
Urine (n=4)
|
97
|
81-106
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Insulin C-Peptide were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
