Human CNTF Quantikine ELISA Kit
Human CNTF Quantikine ELISA Kit
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Human CNTF Quantikine ELISA Kit Summary
货号：DNT00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Heparin Plasma (200 uL), Citrate Plasma (200 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Recombinant human CNTF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human CNTF Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure recombinant human CNTF in cell culture supernates, serum, and plasma. It contains recombinant human CNTF and has been shown to accurately quantitate the recombinant factor.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
110
|
461
|
1295
|
128
|
399
|
821
|
|
Standard Deviation
|
5
|
10.2
|
31.4
|
10.1
|
26.8
|
52.6
|
|
CV%
|
4.5
|
2.2
|
2.4
|
7.9
|
6.7
|
6.4
|
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
103
|
406
|
1201
|
206
|
671
|
1388
|
|
Standard Deviation
|
4.4
|
12
|
28.2
|
19.6
|
47.8
|
93.9
|
|
CV%
|
4.3
|
3
|
2.3
|
9.5
|
7.1
|
6.8
|
Recovery
The recovery of CNTF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
99
|
92-115
|
|
Citrate Plasma (n=5)
|
87
|
71-125
|
|
EDTA Plasma (n=5)
|
90
|
78-97
|
|
Heparin Plasma (n=5)
|
92
|
82-98
|
|
Serum (n=5)
|
95
|
85-101
|
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of CNTF in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
