Human Carbonic Anhydrase IX Quantikine ELISA Kit
Human Carbonic Anhydrase IX Quantikine ELISA Kit
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Human Carbonic Anhydrase IX Quantikine ELISA Kit Summary
货号：DCA900
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human CA9
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Carbonic Anhydrase IX Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human CA9 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human CA9 ectodomain and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CA9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CA9.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
166
|
338
|
673
|
166
|
333
|
647
|
|
Standard Deviation
|
6.36
|
7.19
|
20.5
|
10.5
|
21.6
|
39.7
|
|
CV%
|
3.8
|
2.1
|
3
|
6.3
|
6.5
|
6.1
|
Recovery
The recovery of CA9 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
110
|
103-114
|
|
Citrate Plasma (n=4)
|
96
|
89-111
|
|
EDTA Plasma (n=4)
|
99
|
90-110
|
|
Heparin Plasma (n=4)
|
97
|
88-112
|
|
Serum (n=4)
|
97
|
87-106
|
|
Urine (n=4)
|
101
|
89-114
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CA9 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
