Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit
Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit
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Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit Summary
货号：MRT20
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse and rat TNF RII
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat TNF RII Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat TNF RII in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TNF RII and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant protein accurately. Results obtained using natural mouse or rat TNF RII showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse or rat TNF RII.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
37.2
|
112
|
472
|
38.6
|
119
|
500
|
|
Standard Deviation
|
1.5
|
4.4
|
21
|
1.4
|
3.5
|
14.7
|
|
CV%
|
4
|
3.9
|
4.4
|
3.6
|
2.9
|
2.9
|
Recovery
The recovery of TNF RII spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=5)
|
102
|
83-119
|
|
Mouse EDTA Plasma (n=5)
|
95
|
80-119
|
|
Mouse Serum (n=5)
|
99
|
91-105
|
|
Rat Cell Culture Supernates (n=5)
|
98
|
85-116
|
|
Rat EDTA Plasma (n=5)
|
95
|
83-112
|
|
Rat Serum (n=5)
|
97
|
84-114
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TNF RII were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
