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Human Prolactin Quantikine ELISA Kit
Human Prolactin Quantikine ELISA Kit
Human Prolactin Quantikine ELISA Kit

Human Prolactin Quantikine ELISA Kit

 详情说明

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Human Prolactin Quantikine ELISA Kit Summary

货号:DPRL00

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.264 ng/mL
Assay Range
1.6 - 100 ng/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Prolactin and natural human macroprolactin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human Prolactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Prolactin in serum and plasma. It contains E. coli-expressed recombinant human Prolactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Prolactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Prolactin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 16.3 31.8 53.1 15.4 30.6 54.4
Standard Deviation 0.831 1.24 1.79 0.9 1.48 2.94
CV% 5.1 3.9 3.4 5.8 4.8 5.4

Recovery

The recovery of Prolactin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 94 84-105
Heparin Plasma (n=4) 97 90-104
Serum (n=4) 94 84-108

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Prolactin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Prolactin ELISA Linearity

:DPRL00DPRL00


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