Human Erythropoietin Quantikine ELISA Kit, RUO
Human Erythropoietin Quantikine ELISA Kit, RUO
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Human Erythropoietin Quantikine ELISA Kit, RUO Summary
货号：DEPRU0 规格：96T
Assay Length
4.5 hours (Benchtop Protocol) or 2.5 hours (Shaker Protocol)
Sample Type & Volume Required Per Well
Serum (100 uL), EDTA Plasma (100 uL)
Assay Range
2.5 - 200 mIU/mL (Serum, EDTA Plasma)
Specificity
Natural and recombinant human Epo.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with other substances.
Product Summary
The Quantikine® Human Epo ELISA uses a monoclonal antibody and polyclonal antibody conjugate in a sandwich ELISA format. The assay is designed to measure Epo levels in serum or EDTA plasma in less than 4.5 hours, or less than 2.5 hours using the shaker protocol.
Precision
Benchtop Protocol Precision (Precision within an assay) Four samples of known concentration were tested thirty times to assess intra-assay precision
Shaker Protocol Precision (Precision between assays) Four samples of known concentration were tested in thirty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using three lots of components
Serum, EDTA Plasma
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
|
3
|
4
|
1
|
2
|
3
|
4
|
|
n
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30
|
30
|
30
|
30
|
30
|
30
|
30
|
30
|
|
Mean
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5.82
|
16.01
|
25.18
|
79.02
|
6.45
|
16.22
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26.35
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84.14
|
|
Standard Deviation
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0
|
0
|
0
|
0
|
0
|
0
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0
|
0
|
|
CV%
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8.25
|
4.3
|
5.9
|
4.24
|
10.3
|
3.74
|
4.2
|
3.71
|
Recovery
The recovery of human Epo spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
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Serum (n=)
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|
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Linearity
Five matched serum and plasma specimens containing elevated Epo concentrations were diluted with Specimen Diluent and assayed using the Quantikine Human Epo ELISA. Diluted specimens demonstrated very good linearity when compared to neat concentrations of Epo.
