Mouse Angiopoietin-like 3 Quantikine ELISA Kit
Mouse Angiopoietin-like 3 Quantikine ELISA Kit
Mouse Angiopoietin-like 3 Quantikine ELISA Kit Summary
货号：MANL30 规格：96T
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse ANGPT-L3. This kit detects full-length and 27 kDa N-terminal cleaved mouse ANGPT-L3 but does not detect the 38 kDa C-terminal cleavage fragment.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse ANGPT-L3 immunoassay is a 4.5 hour solid phase ELISA designed to measure ANGPT-L3 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ANGPT-L3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural ANGPT-L3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ANGPT-L3.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
190
|
383
|
1346
|
197
|
418
|
1449
|
|
Standard Deviation
|
10.9
|
23.6
|
79.1
|
17.4
|
27.1
|
90.5
|
|
CV%
|
5.7
|
6.2
|
5.9
|
8.8
|
6.5
|
6.2
|
Recovery
The recovery of ANGPT-L3 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
|
Average % Recovery
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Range %
|
|
Cell Culture Supernates (n=4)
|
103
|
93-120
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of ANGPT-L3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
