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Human Growth Hormone Quantikine ELISA Kit
Human Growth Hormone Quantikine ELISA Kit
Human Growth Hormone Quantikine ELISA Kit

Human Growth Hormone Quantikine ELISA Kit

 详情说明

Human Growth Hormone Quantikine ELISA Kit Summary

Catalog # Availability Size / Price
DGH00         1 Kit / Please Inquire
SGH00         1 Pack (6 Plates) / Please Inquire

PDGH00       1 Pack (50 Plates) / Please Inquire

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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
7.18 pg/mL
Assay Range
25.0 - 1,600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Growth Hormone
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human Growth Hormone Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Growth Hormone in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Growth Hormone and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Growth Hormone showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Growth Hormone.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 201 595 1091 209 623 1152
Standard Deviation 9.4 18.8 26 18.2 45.6 63.6
CV% 4.7 3.2 2.4 8.7 7.3 5.5

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 197 586 1092 215 639 1189
Standard Deviation 8 13.8 29.8 20.2 49.5 82.2
CV% 4.1 2.4 2.7 9.4 7.7 6.9

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