Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit
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Mouse IL-1 alpha/IL-1F1 Quantikine ELISA Kit Summary
货号：MLA00 规格：96T
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL)
Assay Range
4.7 - 300 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-1 alpha
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse IL-1 alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 alpha levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-1 alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-1 alpha accurately. Results obtained using natural mouse IL-1 alpha showed dose response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 alpha.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
17
|
32
|
156
|
18
|
33
|
162
|
|
Standard Deviation
|
1.5
|
1
|
3.9
|
0.9
|
1.2
|
7.9
|
|
CV%
|
8.8
|
3.1
|
2.5
|
5
|
3.6
|
4.9
|
Recovery
The recovery of mouse IL-1 alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=5)
|
102
|
92-109
|
|
Serum (n=6)
|
94
|
88-103
|
Linearity
To assess the linearity of the assay, five or more samples spiked with various concentrations of mouse IL-1 alpha in each matrix were diluted with Calibrator Diluent and then assayed.
