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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
货号：DTRM10C 规格:96T
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Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva
The recovery of human TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Sensitivity
15.2 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human TREM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
479
1226
2217
511
1286
2254
Standard Deviation
16.8
41.5
72.1
25
57.7
90.7
CV%
3.5
3.4
3.3
4.9
4.5
4
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
102
94-112
Cell Lysis Buffer (n=4)
88
78-96
EDTA Plasma (n=4)
99
92-105
Heparin Plasma (n=4)
96
87-104
Serum (n=4)
97
84-107
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human TREM-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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