热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
货号：MSAA00 规格：96T
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Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
The recovery of mouse SAA spiked to levels throughout the range of the assay was evaluated.
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.022 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse SAA.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
0.413
1.35
2.35
0.459
1.35
2.51
Standard Deviation
0.02
0.073
0.11
0.036
0.082
0.178
CV%
4.8
5.4
4.7
7.8
6.1
7.1
Recovery
Sample Type
Average % Recovery
Range %
Culture Media (n=4)
93
84-106
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SAA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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