Mouse GM-CSF Quantikine ELISA Kit
Mouse GM-CSF Quantikine ELISA Kit
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Mouse GM-CSF Quantikine ELISA Kit Summary
Catalog Size / Price
MGM00 1 Kit (2 Plates) / Please Inquire
SMGM00 1 Pack (6 Plates) / Please Inquire
PMGM00 1 Pack (50 Plates) / Please Inquire
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse GM-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse GM-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse GM-CSF in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse GM-CSF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse GM-CSF accurately. Results obtained using natural mouse GM-CSF showed dose-response curves that were parallel to the standard curves obtained using the recombinant standards. These results indicate that this kit can be used to determine relative mass values for natural mouse GM-CSF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
18.5
|
67.2
|
219
|
20
|
71.7
|
243
|
|
Standard Deviation
|
0.7
|
2.1
|
6.9
|
1
|
3.8
|
11.2
|
|
CV%
|
3.8
|
3.1
|
3.2
|
5
|
5.3
|
4.6
|
Recovery
The recovery of mouse GM-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=8)
|
96
|
82-114
|
|
Serum (n=7)
|
94
|
82-112
|
