Human Dkk-1 Quantikine ELISA Kit
Human Dkk-1 Quantikine ELISA Kit
Human Dkk-1 Quantikine ELISA Kit Summary
货号：DKK100
深圳9游总区生物优势代理美国R&DELISA系列产品试剂盒，价格优势，货期短，欢迎来电咨询0755-33164177！
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Dkk-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Dkk-1 immunoassay is a 4.5 hour solid phase ELISA designed to measure human Dkk-1 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human Dkk-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Dkk-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values of natural
human Dkk-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
223
|
600
|
1353
|
221
|
577
|
1287
|
|
Standard Deviation
|
9.4
|
15.3
|
36.5
|
16.9
|
35
|
69.6
|
|
CV%
|
4.2
|
2.6
|
2.7
|
7.7
|
6.1
|
5.4
|
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
212
|
599
|
1358
|
243
|
620
|
1371
|
|
Standard Deviation
|
8.9
|
24.5
|
44.4
|
18.4
|
37.4
|
63.1
|
|
CV%
|
4.2
|
4.1
|
3.3
|
7.3
|
6
|
4.6
|
Recovery
The recovery of human Dkk-1 spiked to various levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
103
|
97-109
|
|
EDTA Plasma (n=4)
|
94
|
86-102
|
|
Heparin Plasma (n=4)
|
98
|
92-106
|
|
Serum (n=4)
|
99
|
85-115
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Dkk-1 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
