Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
Human Fas Ligand/TNFSF6 Quantikine ELISA Kit Summary
货号：DFL00B 规格：96T
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Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Fas Ligand.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Human Fas Ligand/TNFSF6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Fas Ligand.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
92.1
|
240
|
587
|
98.6
|
244
|
576
|
|
Standard Deviation
|
3.31
|
7.51
|
18.2
|
4.41
|
8.7
|
25.7
|
|
CV%
|
3.6
|
3.1
|
3.1
|
4.5
|
3.6
|
4.5
|
Recovery
The recovery of human Fas Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
98
|
93-103
|
|
EDTA Plasma (n=4)
|
103
|
94-111
|
|
Heparin Plasma (n=4)
|
96
|
92-101
|
|
Serum (n=4)
|
101
|
96-103
|
