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Human CXCL9/MIG Quantikine ELISA Kit
Human CXCL9/MIG Quantikine ELISA Kit
Human CXCL9/MIG Quantikine ELISA Kit

Human CXCL9/MIG Quantikine ELISA Kit

 详情说明

Human CXCL9/MIG Quantikine ELISA Kit Summary

货号:DCX900  规格:96T

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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
11.3 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human MIG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human MIG in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MIG.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 235 700 1358 242 707 1391
Standard Deviation 9.5 25.1 24.3 20.8 50.9 83.6
CV% 4 3.6 1.8 8.6 7.2 6

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 253 817 1603 245 723 1427
Standard Deviation 9.9 26.6 49.6 12.8 59.4 88.1
CV% 3.9 3.3 3.1 5.2 8.2 6.2

Recovery

The recovery of MIG spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 86-98
EDTA Plasma (n=5) 100 95-110
Heparin Plasma (n=5) 109 101-114
Serum (n=5) 108 101-113

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