热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
货号：DMP100 规格：96T
Cell Culture Supernates
Serum, Heparin Plasma
The recovery of Pro-MMP-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), Heparin Plasma (100 uL)
Sensitivity
0.095 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant human Pro-MMP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
0.494
1.65
3.39
0.561
1.73
3.5
Standard Deviation
0.022
0.065
0.177
0.065
0.153
0.27
CV%
4.5
4
5.2
11.7
8.8
7.7
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
40
40
40
Mean
0.702
2.13
4.43
0.738
2.34
4.66
Standard Deviation
0.039
0.13
0.241
0.077
0.176
0.312
CV%
5.6
6.1
5.4
10.4
7.5
6.7
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
103
98-112
Heparin Plasma (n=5)
92
85-102
Serum (n=5)
97
87-112
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Pro-MMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
0755-28715175/33164177
