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热门关键词： ELISA试剂盒 - 大鼠ELISA试剂盒- 小鼠ELISA试剂盒
全国统一客服热线：
0755-28715175
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货号：DHD00C 规格：96T
Cell Culture Supernates
Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
The recovery of PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated.
Human PDGF-AB Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Sensitivity
3.83 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Specificity
Natural and recombinant human PDGF-AB
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
92.7
312
656
102
343
701
Standard Deviation
8.28
9.73
15.2
10.9
29.9
52.3
CV%
8.9
3.1
2.3
10.7
8.7
7.5
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
107
350
726
119
362
723
Standard Deviation
6.95
10.9
63.1
10.7
20.9
54.5
CV%
6.5
3.1
8.7
9
5.8
7.5
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
105
93-119
Platelet-poor EDTA Plasma (n=4)
104
78-117
Platelet-poor Heparin Plasma (n=4)
105
86-121
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-AB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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