Mouse IL-2 Quantikine ELISA Kit
Mouse IL-2 Quantikine ELISA Kit
Mouse IL-2 Quantikine ELISA Kit Summary
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Catalog Availability Size
M2000 1 Kit (2 Plates)
SM2000 1 Pack (6 Plates)
PM2000 1 Pack (50 Plates)
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-2 levels in cell culture supernates and serum. It contains E. coli-expressed recombinant mouse IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-2 accurately. Results obtained using natural mouse IL-2 showed dose curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
70.4
|
187
|
595
|
70.9
|
175
|
559
|
|
Standard Deviation
|
3.9
|
10
|
23.4
|
3.6
|
9.6
|
24.5
|
|
CV%
|
5.5
|
5.3
|
3.9
|
5.1
|
5.5
|
4.4
|
Recovery
The recovery of mouse IL-2 spiked to three levels throughout the range of the assay was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
102
|
84-110
|
|
Serum (n=6)
|
102
|
96-108
|
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-2 in each matrix were diluted with Calibrator Diluent and then assayed.
