Human CCL4/MIP-1 beta Quantikine ELISA Kit
Human CCL4/MIP-1 beta Quantikine ELISA Kit
Human CCL4/MIP-1 beta Quantikine ELISA Kit Summary
货号：DMB00
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Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.0 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Citrate Plasma (150 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates), 31.2 - 2,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human MIP-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human MIP-1 beta Immunoassay is a 3.0 or 4.5 hour solid phase ELISA designed to measure MIP-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human MIP-1 beta (Act-2 variant) and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant human MIP-1 beta. Results obtained using natural human MIP-1 beta showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIP-1 beta.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
23.3
|
98.8
|
428
|
31.4
|
118
|
476
|
|
Standard Deviation
|
1.1
|
3.8
|
17.3
|
3.7
|
9.9
|
28
|
|
CV%
|
4.7
|
3.8
|
4
|
11.8
|
8.4
|
5.9
|
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
51.3
|
208
|
932
|
58.8
|
220
|
949
|
|
Standard Deviation
|
4.6
|
7.5
|
30.2
|
5.7
|
15.6
|
70.5
|
|
CV%
|
9
|
3.6
|
3.2
|
9.7
|
7.1
|
7.4
|
Recovery
The recovery of MIP-1 beta spiked to three levels in samples throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
107
|
81-117
|
|
Citrate Plasma (n=5)
|
99
|
87-116
|
|
EDTA Plasma (n=5)
|
100
|
84-112
|
|
Heparin Plasma (n=5)
|
99
|
90-112
|
|
Serum (n=5)
|
99
|
88-113
|
