Rat VEGF Quantikine ELISA Kit
Rat VEGF Quantikine ELISA Kit
Rat VEGF Quantikine ELISA Kit Summary
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货号：RRV00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant rat VEGF. This assay cross-reacts 59% with Mouse VEGF120 (E.Coli-expressed), 73% with Mouse VEGF164 (Sf21-expressed), 14% with Canine VEGF164 (E.Coli-expressed), 26% with Human VEGF121 (Sf21-expressed), 31% with Human VEGF121 (E.Coli-expressed), 24% with Mouse VEGF162 (NS0-expressed), 0.2% with Human VEGF206 (E.Coli-expressed) and 5.2% with Human VEGF/PIGF.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Rat VEGF Immunoassay is a 3.5 hour solid-phase ELISA designed to measure rat VEGF in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat VEGF and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat VEGF.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
71
|
65
|
71
|
|
Mean
|
103
|
191
|
891
|
107
|
233
|
900
|
|
Standard Deviation
|
3.8
|
10.7
|
19.7
|
8.5
|
23.3
|
41.1
|
|
CV%
|
3.7
|
5.6
|
2.2
|
7.9
|
10
|
4.6
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
65
|
60
|
65
|
|
Mean
|
79.4
|
174
|
797
|
87.6
|
204
|
828
|
|
Standard Deviation
|
5.7
|
7
|
14.1
|
8.2
|
21.2
|
42.5
|
|
CV%
|
7.2
|
4
|
1.8
|
9.4
|
10.4
|
5.1
|
Recovery
The recovery of rat VEGF spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=6)
|
103
|
94-108
|
|
EDTA Plasma (n=6)
|
102
|
95-108
|
|
Heparin Plasma (n=6)
|
92
|
80-100
|
|
Serum (n=6)
|
99
|
90-116
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of rat VEGF in each matrix were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
