Human Serpin E1/PAI-1 Quantikine ELISA Kit
Human Serpin E1/PAI-1 Quantikine ELISA Kit
Human Serpin E1/PAI-1 Quantikine ELISA Kit Summary
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货号：DSE100
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), CTAD Plasma (50 uL)
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma)
Specificity
Natural and recombinant human Serpin E1
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human Serpin E1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not detect recombinant human Serpin E1 in complexes with recombinant human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
2.6
|
6.5
|
12.6
|
2.3
|
6.4
|
13.1
|
|
Standard Deviation
|
0.12
|
0.52
|
0.98
|
0.2
|
0.4
|
0.8
|
|
CV%
|
4.4
|
8
|
7.8
|
9.5
|
6.4
|
6.1
|
Recovery
The recovery of Serpin E1 spiked to levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=4)
|
106
|
89-113
|
|
Citrate Plasma (n=4)
|
99
|
88-112
|
|
CTAD Plasma (n=4)
|
100
|
83-112
|
|
EDTA Plasma (n=4)
|
100
|
85-115
|
|
Heparin Plasma (n=4)
|
98
|
85-113
|
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Serpin E1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
