Human Flt-3 Ligand Quantikine ELISA Kit Summary
Human Flt-3 Ligand Quantikine ELISA Kit Summary
Human Flt-3 Ligand Quantikine ELISA Kit Summary
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货号：DFK00
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human Flt-3 Ligand
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Flt-3/Flk-2 Ligand Immunoassay is a 4.0 or 4.5 hour solid phase ELISA designed to measure soluble human Flt-3 Ligand in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Flt-3 Ligand and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Flt-3 Ligand accurately. Results obtained measuring natural human Flt-3 Ligand showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Flt-3 Ligand.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
65.7
|
214
|
421
|
72.8
|
224
|
429
|
|
Standard Deviation
|
1.2
|
4.1
|
9.3
|
6.7
|
13.8
|
30.5
|
|
CV%
|
1.8
|
1.9
|
2.2
|
9.2
|
6.2
|
7.1
|
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
69.5
|
230
|
453
|
78.2
|
241
|
462
|
|
Standard Deviation
|
1.9
|
5.2
|
6.2
|
8.7
|
14.9
|
33.1
|
|
CV%
|
2.7
|
2.3
|
1.4
|
11.1
|
6.2
|
7.2
|
Recovery
The recovery of Flt-3 Ligand spiked to three different levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Media (n=5)
|
102
|
92-118
|
|
Citrate Plasma (n=5)
|
101
|
94-110
|
|
EDTA Plasma (n=5)
|
101
|
90-119
|
|
Heparin Plasma (n=5)
|
100
|
88-112
|
|
Serum (n=5)
|
100
|
91-117
|
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of Flt-3 Ligand in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
