Total MMP-2 Quantikine ELISA Kit Summary
Total MMP-2 Quantikine ELISA Kit Summary
Total MMP-2 Quantikine ELISA Kit Summary
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货号：MMP200
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Assay Range
0.5 - 32 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Recombinant MMP-2 and natural human, mouse, rat, porcine, and canine active, pro-, and TIMP complexed MMP-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Total MMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MMP-2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse/rat pro-MMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural MMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring MMP-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
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Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
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20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
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4.3
|
11.2
|
22.8
|
4.26
|
11.1
|
21.5
|
|
Standard Deviation
|
0.302
|
0.42
|
0.828
|
0.275
|
0.738
|
1.51
|
|
CV%
|
7
|
3.8
|
3.6
|
6.5
|
6.6
|
7
|
Recovery
The recovery of Total MMP-2 spiked to levels throughout the range of the assay was evaluated.
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Sample Type
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Average % Recovery
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Range %
|
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Cell Culture Media (n=4)
|
99
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90-105
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Total MMP-2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
