Mouse IL-5 Quantikine ELISA Kit Summary
Mouse IL-5 Quantikine ELISA Kit Summary
Mouse IL-5 Quantikine ELISA Kit Summary
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货号：M5000
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-5
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-5 in cell culture supernates and mouse serum. It contains Sf 21-expressed recombinant mouse IL-5 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant mouse IL-5. Results obtained using natural mouse IL-5 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-5.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
41.5
|
117
|
428
|
43.4
|
123
|
447
|
|
Standard Deviation
|
2.4
|
3.9
|
8.6
|
1.8
|
5.3
|
20.8
|
|
CV%
|
5.8
|
3.3
|
2
|
4.1
|
4.3
|
4.7
|
Recovery
The recovery of mouse IL-5 spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=5)
|
99
|
85-110
|
|
Serum (n=5)
|
92
|
81-104
|
Linearity
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-5 in each matrix were diluted with Calibrator Diluent and then assayed.
